A unique program for cell death in xylem fibers of Populus stem.
Identifieur interne : 003718 ( Main/Exploration ); précédent : 003717; suivant : 003719A unique program for cell death in xylem fibers of Populus stem.
Auteurs : Charleen L. Courtois-Moreau [Suède] ; Edouard Pesquet ; Andreas Sjödin ; Luis Mu Iz ; Benjamin Bollhöner ; Minako Kaneda ; Lacey Samuels ; Stefan Jansson ; Hannele TuominenSource :
- The Plant journal : for cell and molecular biology [ 1365-313X ] ; 2009.
Descripteurs français
- KwdFr :
- ADN des plantes (analyse), Analyse de profil d'expression de gènes (MeSH), Analyse sur microréseau (MeSH), Microscopie électronique à transmission (MeSH), Mort cellulaire (MeSH), Méthode TUNEL (MeSH), Paroi cellulaire (métabolisme), Populus (génétique), Populus (métabolisme), Régulation de l'expression des gènes végétaux (MeSH), Test des comètes (MeSH), Tiges de plante (cytologie), Tiges de plante (génétique), Tiges de plante (métabolisme), Vacuoles (métabolisme), Xylème (métabolisme).
- MESH :
- analyse : ADN des plantes.
- cytologie : Tiges de plante.
- génétique : Populus, Tiges de plante.
- métabolisme : Paroi cellulaire, Populus, Tiges de plante, Vacuoles, Xylème.
- Analyse de profil d'expression de gènes, Analyse sur microréseau, Microscopie électronique à transmission, Mort cellulaire, Méthode TUNEL, Régulation de l'expression des gènes végétaux, Test des comètes.
English descriptors
- KwdEn :
- Cell Death (MeSH), Cell Wall (metabolism), Comet Assay (MeSH), DNA, Plant (analysis), Gene Expression Profiling (MeSH), Gene Expression Regulation, Plant (MeSH), In Situ Nick-End Labeling (MeSH), Microarray Analysis (MeSH), Microscopy, Electron, Transmission (MeSH), Plant Stems (cytology), Plant Stems (genetics), Plant Stems (metabolism), Populus (genetics), Populus (metabolism), Vacuoles (metabolism), Xylem (metabolism).
- MESH :
- chemical , analysis : DNA, Plant.
- cytology : Plant Stems.
- genetics : Plant Stems, Populus.
- metabolism : Cell Wall, Plant Stems, Populus, Vacuoles, Xylem.
- Cell Death, Comet Assay, Gene Expression Profiling, Gene Expression Regulation, Plant, In Situ Nick-End Labeling, Microarray Analysis, Microscopy, Electron, Transmission.
Abstract
Maturation of the xylem elements involves extensive deposition of secondary cell-wall material and autolytic processes resulting in cell death. We describe here a unique type of cell-death program in xylem fibers of hybrid aspen (Populus tremula x P. tremuloides) stems, including gradual degradative processes in both the nucleus and cytoplasm concurrently with the phase of active cell-wall deposition. Nuclear DNA integrity, as determined by TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) and Comet (single-cell gel electrophoresis) assays, was compromised early during fiber maturation. In addition, degradation of the cytoplasmic contents, as detected by electron microscopy of samples fixed by high-pressure freezing/freeze substitution (HPF-FS), was gradual and resulted in complete loss of the cytoplasmic contents well before the loss of vacuolar integrity, which is considered to be the moment of death. This type of cell death differs significantly from that seen in xylem vessels. The loss of vacuolar integrity, which is thought to initiate cell degradative processes in the xylem vessels, is one of the last processes to occur before the final autolysis of the remaining cell contents in xylem fibers. High-resolution microarray analysis in the vascular tissues of Populus stem, combined with in silico analysis of publicly available data repositories, suggests the involvement of several previously uncharacterized transcription factors, ethylene, sphingolipids and light signaling as well as autophagy in the control of fiber cell death.
DOI: 10.1111/j.1365-313X.2008.03777.x
PubMed: 19175765
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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comètes (MeSH)</term><term>Tiges de plante (cytologie)</term><term>Tiges de plante (génétique)</term><term>Tiges de plante (métabolisme)</term><term>Vacuoles (métabolisme)</term><term>Xylème (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA, Plant</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN des plantes</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Tiges de plante</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Plant Stems</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plant Stems</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Populus</term><term>Tiges de plante</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Wall</term><term>Plant 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xylem elements involves extensive deposition of secondary cell-wall material and autolytic processes resulting in cell death. We describe here a unique type of cell-death program in xylem fibers of hybrid aspen (Populus tremula x P. tremuloides) stems, including gradual degradative processes in both the nucleus and cytoplasm concurrently with the phase of active cell-wall deposition. Nuclear DNA integrity, as determined by TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) and Comet (single-cell gel electrophoresis) assays, was compromised early during fiber maturation. In addition, degradation of the cytoplasmic contents, as detected by electron microscopy of samples fixed by high-pressure freezing/freeze substitution (HPF-FS), was gradual and resulted in complete loss of the cytoplasmic contents well before the loss of vacuolar integrity, which is considered to be the moment of death. This type of cell death differs significantly from that seen in xylem vessels. The loss of vacuolar integrity, which is thought to initiate cell degradative processes in the xylem vessels, is one of the last processes to occur before the final autolysis of the remaining cell contents in xylem fibers. High-resolution microarray analysis in the vascular tissues of Populus stem, combined with in silico analysis of publicly available data repositories, suggests the involvement of several previously uncharacterized transcription factors, ethylene, sphingolipids and light signaling as well as autophagy in the control of fiber cell death.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19175765</PMID><DateCompleted><Year>2009</Year><Month>05</Month><Day>28</Day></DateCompleted><DateRevised><Year>2009</Year><Month>05</Month><Day>05</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1365-313X</ISSN><JournalIssue CitedMedium="Internet"><Volume>58</Volume><Issue>2</Issue><PubDate><Year>2009</Year><Month>Apr</Month></PubDate></JournalIssue><Title>The Plant journal : for cell and molecular biology</Title><ISOAbbreviation>Plant J</ISOAbbreviation></Journal><ArticleTitle>A unique program for cell death in xylem fibers of Populus stem.</ArticleTitle><Pagination><MedlinePgn>260-74</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/j.1365-313X.2008.03777.x</ELocationID><Abstract><AbstractText>Maturation of the xylem elements involves extensive deposition of secondary cell-wall material and autolytic processes resulting in cell death. We describe here a unique type of cell-death program in xylem fibers of hybrid aspen (Populus tremula x P. tremuloides) stems, including gradual degradative processes in both the nucleus and cytoplasm concurrently with the phase of active cell-wall deposition. Nuclear DNA integrity, as determined by TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) and Comet (single-cell gel electrophoresis) assays, was compromised early during fiber maturation. In addition, degradation of the cytoplasmic contents, as detected by electron microscopy of samples fixed by high-pressure freezing/freeze substitution (HPF-FS), was gradual and resulted in complete loss of the cytoplasmic contents well before the loss of vacuolar integrity, which is considered to be the moment of death. This type of cell death differs significantly from that seen in xylem vessels. The loss of vacuolar integrity, which is thought to initiate cell degradative processes in the xylem vessels, is one of the last processes to occur before the final autolysis of the remaining cell contents in xylem fibers. High-resolution microarray analysis in the vascular tissues of Populus stem, combined with in silico analysis of publicly available data repositories, suggests the involvement of several previously uncharacterized transcription factors, ethylene, sphingolipids and light signaling as well as autophagy in the control of fiber cell death.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Courtois-Moreau</LastName><ForeName>Charleen L</ForeName><Initials>CL</Initials><AffiliationInfo><Affiliation>Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, 90187 Umeå, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pesquet</LastName><ForeName>Edouard</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Sjödin</LastName><ForeName>Andreas</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Muñiz</LastName><ForeName>Luis</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Bollhöner</LastName><ForeName>Benjamin</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Kaneda</LastName><ForeName>Minako</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Samuels</LastName><ForeName>Lacey</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Jansson</LastName><ForeName>Stefan</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Tuominen</LastName><ForeName>Hannele</ForeName><Initials>H</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2008</Year><Month>12</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Plant J</MedlineTA><NlmUniqueID>9207397</NlmUniqueID><ISSNLinking>0960-7412</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018744">DNA, Plant</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D016923" MajorTopicYN="Y">Cell Death</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020552" MajorTopicYN="N">Comet Assay</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018744" MajorTopicYN="N">DNA, Plant</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020869" MajorTopicYN="N">Gene Expression Profiling</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020287" MajorTopicYN="N">In Situ Nick-End Labeling</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D046228" MajorTopicYN="N">Microarray Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D046529" MajorTopicYN="N">Microscopy, Electron, Transmission</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018547" MajorTopicYN="N">Plant Stems</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014617" MajorTopicYN="N">Vacuoles</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D052584" MajorTopicYN="N">Xylem</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>1</Month><Day>30</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>1</Month><Day>30</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>5</Month><Day>29</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19175765</ArticleId><ArticleId IdType="pii">TPJ3777</ArticleId><ArticleId IdType="doi">10.1111/j.1365-313X.2008.03777.x</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Suède</li></country></list><tree><noCountry><name sortKey="Bollhoner, Benjamin" sort="Bollhoner, Benjamin" uniqKey="Bollhoner B" first="Benjamin" last="Bollhöner">Benjamin Bollhöner</name><name sortKey="Jansson, Stefan" sort="Jansson, Stefan" uniqKey="Jansson S" first="Stefan" last="Jansson">Stefan Jansson</name><name sortKey="Kaneda, Minako" sort="Kaneda, Minako" uniqKey="Kaneda M" first="Minako" last="Kaneda">Minako Kaneda</name><name sortKey="Mu Iz, Luis" sort="Mu Iz, Luis" uniqKey="Mu Iz L" first="Luis" last="Mu Iz">Luis Mu Iz</name><name sortKey="Pesquet, Edouard" sort="Pesquet, Edouard" uniqKey="Pesquet E" first="Edouard" last="Pesquet">Edouard Pesquet</name><name sortKey="Samuels, Lacey" sort="Samuels, Lacey" uniqKey="Samuels L" first="Lacey" last="Samuels">Lacey Samuels</name><name sortKey="Sjodin, Andreas" sort="Sjodin, Andreas" uniqKey="Sjodin A" first="Andreas" last="Sjödin">Andreas Sjödin</name><name sortKey="Tuominen, Hannele" sort="Tuominen, Hannele" uniqKey="Tuominen H" first="Hannele" last="Tuominen">Hannele Tuominen</name></noCountry><country name="Suède"><noRegion><name sortKey="Courtois Moreau, Charleen L" sort="Courtois Moreau, Charleen L" uniqKey="Courtois Moreau C" first="Charleen L" last="Courtois-Moreau">Charleen L. Courtois-Moreau</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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